Biiogeneic

Recombinant DNA Technology
	Introduction, Calculation, Preparation of Buffers and Reagents
	Preparation of Media inoculation and growth of test organisms.
	Isolation of Genomic DNA from Plant
	Isolation of DNA from Yeast
	Purification of the extracted DNA
	Quantitative and Qualitative estimation of DNA
		By Agarose gel electrophoresis
		Colorimetric method using DPA
		Spectrophotometric method
	Isolation of Plasmid DNA by alkali lysis method
	Agarose gel electrophoresis
	Elution of DNA from the gel
	Restriction digestion & gel electrophoresis
	Ligation and gel electrophoresis
	Conjugation
	Southern Blotting
	UV Sensitivity of E.Coli