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Techniques in Plant tissue culture
Culture media (Nutrition media)
  Macronutrients
  Micronutrients
  Organic Supplements-vitamin, growth regulators
  Carbon source
  Gelling agents
pH
Selection of explants
Maintenance of aseptic conditions
  Sterilization of glassware & plant material
Preparation of stock solutions and media
Callus culture
Embryo culture
Meristem culture
  Root culture
  Anther culture
  Shoot tip culture
  Leaf disc culture
Suspension culture
Synthetic seed production
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