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| P.G. Diploma in Biotechnology |
| Introduction |
Biotechnology is a multi disciplinary science comprising various facets of Biology. This course provides a advanced theoretical and practical aspects required in a Biotech Industry. |
| Course Structure (Theory): |
| Module I Fundamentals |
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Bio Chemistry and Analytical Bio Chemistry |
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Enzymologyand Enzyme Technology |
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Analytical Bio Chemistry in Biology |
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Computers Applications and Bio Statistics |
| Module II Molecular Biology |
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Cell Biology |
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Molecular Genetics |
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Recombinant DNA Technology |
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Genetic Engineering |
| Module III Environmental Biotechnology |
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Microbiology |
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Microbial Technology |
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Fermentation and Bioprocessing |
| Module IV Plant and Animal Biotechnology |
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PlantTissue Culture |
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Animal Cell Culture Methods |
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Immunologyand Immuno Technology |
| Module V Project Dissertation |
| Course Structure (Practical): |
| Module I Industrial Microbiology |
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Isolation of Micro Organism from natural sources |
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Identification of Bacteria |
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Sterility Techniques |
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Bacterial Growth Curve |
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Mutation Studies |
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Screening of Micro Organism for Industrial Applications |
| Module II Enzymologyand Protein Chemistry |
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Identification of Enzyme Activity |
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Qualification of Proteins |
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Purification of Enzymes |
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Chemical Techniques |
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Physical Column Chromatography |
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Characterization of Enzymes |
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SDSPage |
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pH, Temperature Optimization |
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Enzyme Kinetics |
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Enzyme Formulation and Stabilization |
| Module lll Molecular Biology & Genetic Engineering |
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Isolation of DNA |
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Transformation in E.coli |
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Isolation of Plasmids |
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Gel Electrophoresis |
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Restriction and Digestion of DNA |
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Ligation Reactions and Cloning |
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Southern Blotting and Hybridization |
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Western Blotting |
| Module lV Immunology |
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Raising Polyclonal Antibodies |
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Purification of lgG/SDS-Page |
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Serum Electrophoresis |
| Module V Protein Analysis |
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HPLC Analysis |
| Fundamentals Programme: |
All Aspects of Basic Programme plus independently operating the HPLC Systems and allied equipment as well as independently performing various operation in HPLC Analysis including preparation of mobile phase, cleaning of pre-filter/injector, attaching column, preparation of solution for analysis, injecting the samples/standard solutions, recording, storage and retrieval of analysis data etc. |
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GLC Analysis |
| Fundamentals Programme: |
All Aspects of Basic Programme plus independently operating the GLC System and allied equipment as well as independently performing various operation in GLC Analysis including attaching SS Column, setting carrier gas flow, operating GC Controls, preparation/injection of samples, recording, storage and retrieval of analysis data etc. |
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