Biiogeneic

Enzymology and Protein Chemistry
	Preparation and sterilization of media
	Isolation of microorganisms from soil
	Screening of microorganism for production of enzyme of Industrial importance
	Optimization of media for enzyme production
	Isolation of a- amylase from potato or saliva
	Quantitative estimation of protein by Lowry's method
	Assay of enzyme activity
	Purification of enzyme
		Ammonium sulfate precipitation method
		Column Chromatography
	Dialysis
	Qualitative estimation of enzyme
		SDS PAGE
		Native PAGE
	Enzyme kinetics
		Effect of PH on enzyme activity
		Effect of Temperature on Enzyme activity
		Effect of Substrate concentration
		Effect of metal ions on enzyme activity
	Enzyme Immobilization
	Assay of Immobilized enzymes
	Determination of Invertase activity
	Determination of Protease activity by Aspergillus species
	Spectrophotometric assay of Lactate dehydrogenase
	Determination of lysozyme activity
	Effect of inhibitors on enzyme activity
	Detection of proteins
		Western blotting
		ELISA (micro titer plate method)
	Qualitative test for proteins
		Biuret test
		Ammonium sulfate precipitation test
		Sulphosalicylic acid test
		Heat coagulation test
	Determination of proteins by Biuret method
	Isolation of Casein from milk by isoelectric precipitation
	Measurement of enzyme activity
		Acid Phosphatase method
		Assay of b-amylase
	Staining technique
		Coomasive Brilliant blue strain R 250