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| Diploma in Enzyme Biotechnology and Protein Purification |
| Introduction |
| Enzymes are biological catalysts, regulating the entire life processes. A detailed knowledge of enzyme Biotechnology is a pre requisite for success in Commercial Biotechnology, as enzyme occupy bulk share of global Biotech Business. This course provides a detailed insight into practical domain of Enzymes and Proteins. |
| Course Structure |
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Protein Estimation |
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Lowry's method |
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Bradford method |
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Enzyme Assays |
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Spectrophotometer |
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Isolation of enzymes from natural sources |
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Identification of enzyme activity |
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Enzyme Purification |
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Precipitation techniques |
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Column chromatography |
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Ion exchange |
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Enzyme analysis |
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Electrophoresis |
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SDS-PAGE |
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Determination of Isoelectric point |
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Enzyme Kinetics |
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Optimization of pH, temperature |
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Activators and inhibitors |
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Enzyme Immobilization Techniques |
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