Biiogeneic

Clinical And Pharmaceutical Microbiology
Clinical microbiology
	Preparation of different types of media and sterilization
	Culturing methods and identification of Microorganisms from
		Blood
		Urine
		Stools
		Pus
		Wound
		Hair
		Nail etc.
	Specific media for identification of pathogenic microorganisms.
		E.coli
		Staphylococcus aureus
		Salmonella typhi
		Pseudomonas aeruginosa
	Biochemical identification
	IMVIC
	Coagulase
	Starch hydrolysis
	TSI.
	Oxidase
	Catalase
	Identification of tubercle bacilli from sputum sample
		Zheil nelson
	Bacterial growth curve.
	Detection of MIC, MBC.
	Antibiotic sensitivity.
	Personal hygiene monitoring.
	Serology.
		WIDAL Test
		Blood Grouping
		VDRL Test
	MP Smear.
	Collection and Processing of Blood, Urine and Stool samples.
	Development of Selective Resistant mutants by gradient concentration method.
	Detection of Antibacterial/Antibiotic competency-Protein binding in blood.
Pharmaceutical microbiology
	Water analysis-USP.
	Bioload monitoring-BP.
	Bacterial Endotoxin tests-USP.
	Pathogen testing-USP.
	Antibiotic assay-USP.
	Vitamin assay-USP, IP.
	Lactobacillus assay-IP.
	Disinfectant efficacy testing-USP.
	Preservative efficacy testing-USP.
	Sterility studies-USP, BP, EP.
	Anaerobic cultivation of Bacteria-USP.
	Environmental effects on Microbial growth.
		Determination of optimal temperature.
		Determination of TDT and Thermal death rate.
		Effect of pH on growth.
		Effect of U.V. on growth.
		Effect of salt concentration
	Prerequisites in simulation trial - with special priority to microbiological attributes.
		Personnel.
		Machines.
		Products.
		Air monitoring.
		Process.
	Validation studies.
		Method validation
		Process validation
		Cleaning validation
		Equipment validation
	Trend analysis
		Air monitoring
		Water monitoring
	Documentation
		SOP
		STP