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| Diploma in General Biotechnology |
| Introduction |
Biotechnology is a multi-disciplinary science comprising various facets of Biology. This course provides an overall view of practical aspects of Biotech Industry. |
| Course Structure |
| Module I Industrial Micro Biology |
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Sterility Techniques |
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Isolation of Micro - Organisms from Various Sources |
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Identification of Bacteria |
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Staining |
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Bio Chemical Tests |
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Bacterial Growth Curve |
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Mutation Studies |
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Screening of Micro Organisms for Industrial Applications |
| Module II Enzymology and Protein Chemistry |
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Assay of Enzyme Activity |
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Quantification of Proteins |
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Purification of Enzymes |
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Chemical Techniques |
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Physical-Column Chromatography |
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Characterization of Proteins |
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SDSPage |
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Enzyme Kinetics |
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pH |
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Temperature |
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Km&Vmax |
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Salt inhibition |
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Enzyme Formulation and Stabilization |
| Module lll Molecular Biology and Genetic Engineering |
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Isolation of DNA&RNAfromEukaryotic and Prokaryotic Source |
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Transformation in E.coM |
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Isolation of Plasmids |
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Gel Electrophoresis |
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Restriction & Digestion of DNA |
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Ligation Reactions & Cloning |
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Southern Blotting and Hybridisation |
| Module IV Bio-Physical Chemistry |
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Chromatography Techniques: |
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HPLCAnalysis |
| Fundamental Programme: |
| All aspects of Basic Programme plus independently operating the HPLC System and allied equipment as well as independently performing various operation in HPLC analysis including preparation of mobile phase, cleaning of p re-filter/injector, attaching column, preparation of solution for analysis, injecting the samples/standard solutions, recording, storage and retrieval of analysis data etc. |
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GLCAnalysis |
| Fundamental Programme: |
| All aspects of Basic Programme plus independently operating the GLC System and allied equipment as well as independently performing various operation in GLC analysis including attaching SS column, setting carrier gas flow, operating GC controls, preparation / injection of samples, recording, storage and retrieval of analysis data etc. |
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