The greatest impact of Biotechnology has been in Health Care and Diagnostics. Knowledge of Pharmaceutical Biotechnology is an indisputable tool for aspiring Pharmacy Professionals, Doctors and Biotechnologists. The course aims at providing sufficient practical training and exposure to Pharmaceutical Biotechnology.
Course Structure
Module I Pharmaceutical Microbiology
Isolation and Preservation of Micro Organisms from natural sources
Identification of Bacteria
Staining
Simple
Differential
Acid fast
Flagella
Capsule
Spore
Motility of Bacteria by hanging drop method
Bio Chemical Tests
Sterility Techniques
Mediasused
Techniques employed
Different material to be sterilised
Bacterial Growth Curve
Mutation Studies
Screening of Micro Organisms for Industrial Applications
Screening for Antibiotic Resistance Microbes
Isolation of Clinically Important Microflora
Antibiotic Assays using Micro Organisms
Sensitivity Testing
Phenol Coefficient Test
Strains Improvement and their detailed treatment
Counting of Bacteria
Cultivation of Virus
Module II Genetic Engineering
Isolation of NDA
Transformation in E.coli
Gel Electrophoresis
Restriction and Digestion of DNA
LigationReactions and Cloning
Southern Blotting
PCR Recombinant DNATechnique
Module lll Pharmaceutical Enzymology & Protein Chemisty
Identification of Enzyme Activity
Quantification of Proteins
Purification of Enzymes
Chemical Techniques
Physical-Column Chromatography
Characterization of Enzymes
SDSPage
pH, Temperature, Optimization
Enzyme Kinetics
Demonstration of Chemotherapeutic effect for anti inflammation /wound healing
Module lV Immunology
Antigen Antibody Reactions
Agglutination
ELISA AIITypes
Immuno Electrophoresis
Serum Proteins, Separations and Purification
Serum Chemistry
Module VBio-Physical Chemistry
GLCAnalysis
HPLCAnalysis
Module VI Case Studies of the following:
Production of Insulin by Recombinant DNATechnique
Production of Hepatitis Vaccine Recombinant DNATechnique
Production of Growth Hormone Recombinant DNATechnique
Production of Vaccine by Recombinant DNATechnique
Monoclonal Antibodies and their Importance in Drug Delivery, Pathological Laboratories etc.
HACCP
Module VII Animal Tissue Culture
Requirements for Animal Tissue Culture
Nutrient Recruitments
Methods adapted forthe same
Application of Animal Tissue Culture in formulation development with an example
Module VIII Drug Delivery Systems
NDDS
CD
SR Pattern
Module IX Standardisation of Natural Extracts
Extraction and Purification of Anti Microbial from Natural Sources
MIC
Module X statutory Quality Standards for the Pharma Industry
